Class 12 Biology MCQ – Biotechnology and its Applications

1. Which of the following is NOT an application of biotechnology?

a) Producing synthetic polymers
b) Genetically modified crops
c) Gene therapy
d) Biofuel production

Answer:

a) Producing synthetic polymers

Explanation:

While synthetic polymers have numerous applications, their production is not directly linked to biotechnology.

2. Bt cotton is genetically modified to:

a) Improve its color
b) Increase its length
c) Resist insect pests
d) Increase its softness

Answer:

c) Resist insect pests

Explanation:

Bt cotton has been modified with a gene from the bacterium Bacillus thuringiensis to produce a protein toxic to certain pests.

3. Golden rice is genetically modified to:

a) Improve its taste
b) Increase its yield
c) Resist herbicides
d) Produce vitamin A

Answer:

d) Produce vitamin A

Explanation:

Golden rice is engineered to produce beta-carotene, a precursor of vitamin A, to combat vitamin A deficiency.

4. Which technique is used for producing genetically identical animals?

a) Cloning
b) Fertilization
c) Crossing over
d) Mutation

Answer:

a) Cloning

Explanation:

Cloning produces genetically identical organisms, typically by transferring the nucleus of a donor adult cell to an egg without a nucleus.

5. The first cloned animal was a:

a) Mouse
b) Cow
c) Sheep
d) Goat

Answer:

c) Sheep

Explanation:

The first cloned mammal was Dolly the sheep, produced in 1996.

6. Transgenic animals are those:

a) Born through in-vitro fertilization
b) Having foreign genes inserted into their DNA
c) Produced by cross-breeding different species
d) Born through natural selection

Answer:

b) Having foreign genes inserted into their DNA

Explanation:

Transgenic animals have had foreign genes deliberately inserted into their genome.

7. Gene therapy aims to:

a) Improve physical appearance
b) Increase lifespan by 50 years
c) Correct genetic disorders
d) Enhance cognitive abilities

Answer:

c) Correct genetic disorders

Explanation:

Gene therapy seeks to treat genetic diseases by introducing, altering, or suppressing a gene.

8. Which of the following is a biopesticide?

a) DDT
b) Neem extract
c) Roundup
d) Malathion

Answer:

b) Neem extract

Explanation:

Neem extract, derived from the neem tree, acts as a natural insect repellent and pesticide.

9. RNA interference has been used in plants to:

a) Make them glow in the dark
b) Improve their nutrient content
c) Develop resistance against specific viruses
d) Increase their height

Answer:

c) Develop resistance against specific viruses

Explanation:

RNA interference (RNAi) silences specific genes and has been utilized to impart virus resistance in plants.

10. The primary reason for creating genetically modified organisms (GMOs) is to:

a) Speed up their growth
b) Make them more aesthetically pleasing
c) Increase their market price
d) Introduce specific beneficial traits

Answer:

d) Introduce specific beneficial traits

Explanation:

GMOs are primarily created to introduce beneficial traits such as disease resistance, increased nutritional value, or improved yield.

11. Which organism is used to produce human insulin using recombinant DNA technology?

a) Horse
b) E. coli
c) Yeast
d) Goat

Answer:

b) E. coli

Explanation:

The bacterium E. coli has been genetically modified to produce human insulin for therapeutic use.

12. Which enzyme is crucial for the amplification of a specific DNA sequence in PCR?

a) Restriction enzyme
b) DNA ligase
c) Taq polymerase
d) DNA gyrase

Answer:

c) Taq polymerase

Explanation:

Taq polymerase is a DNA polymerase enzyme used in PCR to amplify specific DNA sequences.

13. Cry genes, which are used to develop Bt crops, originate from which bacterium?

a) Escherichia coli
b) Rhizobium
c) Bacillus thuringiensis
d) Agrobacterium tumefaciens

Answer:

c) Bacillus thuringiensis

Explanation:

Cry genes, sourced from Bacillus thuringiensis, encode for proteins toxic to certain insect pests.

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